Device

Part:BBa_K2360015:Design

Designed by: CHEN NANZHU   Group: iGEM17_SCUT-China_A   (2017-10-27)


rrnB' terminater+PbrR+pPbrR+GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1446


Design Notes

We use the standard biobrick to make this part.


Source

We combined the GFP module (BBa_E0240) and mercury detecting module((BBa_K2360006) to make this part.

References

[1]. Lara Bereza-Malcolm, Sanja Aracic and Ashley E. Franks. Development and Application of a Synthetically-Derived Lead Biosensor Construct for Use in Gram-Negative Bacteria [J]. Sensors (Basel). 2016 Dec 18;16(12).
[2]. Leveau J H, Lindow S E. Predictive and interpretive simulation of green fluorescent protein expression in reporter bacteria[J]. Journal of Bacteriology, 2001, 183(23):6752.